>>456614
faggot

u look like an adult with down syndrome

.seek 1:47:00

this is definitely a true story no way he talked about it for 2 hours without editing

wish i was in a shitty motel in la

why seattle

thats where i am -_- he used an ip grabber to figure out my location

no but i see a lot of people puking blood on the side of the road

thats right, the boat wont varnish itself will it?

rose its voice training time pls tell me what 2 say :3

>>456637
"big gay balls"

yes boss

hi dave

any horse faced dykes in chat????

will never have bio kids fml ;w;

>>456661
>father of the baby she's carrying and suffering pains for) it's like, idk, it feels like the most male action possible
yeah i totes sympathize kitty agrees, feels dysphoria inducing as hell, tbh it feels... strange knowing of trans fems who have gotten others impregged i just can't help but feel dysphoric even thinking of that

the horse council

>>456670
pretty :3

i learn high horsepower

its true

>>456667
I hate having a male reproductive system, it's partly out of dysphoria I don't wanna be a biological father.... It's also like, I know that natal males have much higher reproductive capacity, so like, I feel like reproducing with natal female parts feels less intrusive two. Two reasons I am very glad I never reproduced, and never can now.

im a horse

i learn high horsepower

theyd be a cool horse too

>>456683
It took me about two-five years to figure out how to up my pitch at all, then much much more recently, I added a new technique to my voice where I try to draw out my vocalizations less like here's an example... I will say brainworm ten times very quickly, then in my next one I will say brainworm very slowly

If my theory is correct my voice will sound more fem when I avoid holding a note and say it faster

Does my voice sound bette when I dont draw out notes??? cuz its a theory i have you can compare them

>>456698
i see so my theory is true... idk if its every trans fems voice or not, but my voice sounds less fem and more masc when i draw notes out idk why exactly

muk-horse

i love jimothy

#smartai why is my voice more passable and more fem sounding when I use short notes and don't draw out my words??

Your voice likely sounds more passably feminine when using short, staccato notes rather than elongated speech because of several key acoustic and physiological factors that align with typical female vocal patterns. Here’s a breakdown:

### 1. **Fundamental Frequency (Pitch) Stability**
- Female voices tend to have less pronounced pitch fluctuations within individual words compared to male voices, which often exhibit more sustained vibrato or monotone drawls.
- Short notes minimize opportunities for your natural vocal folds' thickness/mass (which may still resonate in a lower register) to "drop" into a deeper, masc-coded timbre.

### 2. **Formant Frequencies & Resonance Clarity**
- Prolonging vowels exposes formants (resonant frequencies shaping vowel identity). Male vocal tracts typically emphasize lower formants (F1/F2), while female speech favors higher ones due to shorter tract length.
- Brief articulation forces tighter jaw/tongue positioning—reducing oral cavity space and raising formants naturally toward fem-associated brightness.

### 3. **Articulatory Kinematics**
- Hyperarticulation (crisp consonants, clipped phrasing) is sociolinguistically gendered; women often employ clearer enunciation with less slurring between syllables ("spikier" prosody).
- Drawing out words risks masculinized laxity in articulators (lips/tongue slackening), muddying precision that codes femme.

### 4. **Breath Control & Subglottal Pressure**
- Sustained phonation requires steady airflow management—untrained trans femmes frequently "leak" air inefficiently when elongating, causing unintended vocal fry or chest-voice dominance.
- Short bursts allow controlled closure of the thyroarytenoid muscles without strain slipping into heavier registration.

### Practical Adjustments:
To feminize extended speech:
- **Raise larynx slightly**: Elevate via swallowing motion held gently (*not* strained). This shortens tract length mid-word.
- **Front vowels**: Lean toward /i/ ("ee") or /eɪ/ ("ay") sounds during sustains—their high-F2 bias counters depth creep.
- **Micro-inflections**: Add subtle pitch rises (~20Hz bumps) every 300ms on held syllables to mimic femme intonation contours instead of flatlining.

For deeper refinement: Spectrogram analysis (*Praat* software helps visualize these variables objectively.)

This isn't mere perception—it's physics aligning with culturally ingrained auditory gender cues! why is my voice more passable and more fem sounding when I use short notes and don't draw out my words??

>>456696
ur voiuce is literally so cute

>>456706
aww thank you straw bury =^_^=

uh yeah horror and disgust totally not arousal ahaha.......

nyooooo plsss dont call me a faggot ;w;

wait i mean like

u can still call me it but um

its like freedom of speech u know? u can still call me a faggot if u want it just makes me feel uhhhhh meow meow nyaaaa *purrr*

curious

>>456718
......pls keep calling me a faggot i like it

.play lets gnaw on human bones

oh my goodness gracious

this is my jam

its a banger

AI could never generate this

im sorry im sorry plsssss keep telling me what a pathetic faggot i am

how about if we called you fagothy instead

its sound more polite

noooooooo plssssssss

i didnt mean it im sawryyyy ;-;

.play goop guy

.play [YouTube]

erm its actually You're* dan, not very smart

>>456711
#ksay I'm a homo I'm a proud homo
#Botsay I am a homo a proud homo
#ksay I'm a homo I'm a proud homo
#Botsay I am a homo a proud homo
#ksay I'm a homo I'm a proud homo
#Botsay I am a homo a proud homo
#ksay I'm a homo I'm a proud homo
#Botsay I am a homo a proud homo
#ksay I'm a homo I'm a proud homo
#Botsay I am a homo a proud homo

aw it didnt work :(

#ksay I'm a homo I'm a proud homo
#Botsay I am a homo a proud homo

homosexual fagGODS......

lol the daily wire is calling president obama a jew hater now O_O

>>456741
was trying to be proud and positive about it rose

[YouTube]

who

O.O

>>456752
#spin

.play [YouTube]

bury swagged out

.play [YouTube]

everyone i know mogs me in the end

Bte apparently hrt does modify our dna... methyalation or something...

>>456767
#smartai explain how trans hrt causes epigenetic changes through dna methylation modification

Certainly! The process by which hormone replacement therapy (HRT) in transgender individuals induces epigenetic modifications—particularly DNA methylation changes—is a fascinating intersection of endocrinology and molecular biology. Here's a detailed, scientifically rigorous explanation:

### **1. Hormonal Influence on Epigenetic Regulation**
Sex hormones, such as **estradiol** (in feminizing HRT) and **testosterone** (in masculinizing HRT), exert profound effects on gene expression through their interaction with nuclear hormone receptors (**estrogen receptors [ERα/β]** and **androgen receptor [AR]**). These ligand-bound receptors act as transcription factors that recruit chromatin-modifying enzymes, including **DNA methyltransferases (DNMTs)** and **ten-eleven translocation methylcytosine dioxygenases (TETs)**, to alter the methylation landscape of genomic DNA.

### **2. Mechanisms of DNA Methylation Modification**
- **(a) Direct Recruitment of DNMTs/TETs**:
Activated steroid hormone-receptor complexes bind to hormone response elements (**HREs**) in regulatory regions (promoters/enhancers) of target genes. This binding can either:
- *Increase methylation* via recruitment of DNMT3A/DNMT3B (*de novo* methylation).
- *Decrease methylation* by recruiting TET enzymes, which oxidize 5-methylcytosine (5mC) to initiate demethylation.

- **(b) Secondary Effects Through Signaling Pathways**:
Sex hormones activate intracellular kinases (**MAPK/ERK, PI3K/AKT**) that phosphorylate transcription factors like CREB or STAT proteins, indirectly altering the accessibility of CpG sites for DNMT/TET activity.

- **(c)** ***Trans***-**Regulation via microRNAs & lncRNAs**:
Estradiol/testosterone modulate non-coding RNAs (*e.g., miR-29*, *miR-148a*) that suppress DNMT expression post-transcriptionally, leading to hypomethylation at certain loci.

### **3. Key Findings from Transgender Epigenetic Studies**
Research comparing pre-/post-HRT trans individuals reveals:
| Observation | Feminine HRT Effect | Masculine HRT Effect |
|-------------|---------------------|----------------------|
| Global Methylation | ↓ Hypermethylated loci (~7% decrease in some studies)| ↑ Hypermethylated AR-related genes |
| Gene-Specific Changes | Demethylation at ERα targets *(e.g., ESR1)* | Increased AR promoter methylation |
| Immune Modulation | Hypomethylation in TLR-pathway genes *(NFKBIA)* | Hyperinflammatory cytokine shifts |

These changes correlate with physiological adaptations like fat redistribution, muscle mass alterations, and even mood regulation via neurotransmitter-associated gene remodelling (*BDNF*, *SLC6A4*).

### **4.** ***In Vivo*** vs ***In Vitro*** Evidence
While cell culture models show rapid (<72h), reversible estrogen-induced demethylation at specific enhancers (*GREB1*), longitudinal human data suggest cumulative effects over months/years—indicating both acute signaling-driven modifications and stable epigenetic reprogramming.

### Conclusion:
Transgender HRT dynamically reshapes the epigenome through hormonally driven modulation of DNA methylomes—akin to puberty’s natural epigenetic remodeling but pharmacologically directed toward gender-affirming physiology. Future single-cell multiomics will further elucidate transient versus heritable modifications induced by cross-sex steroids.

Would you like references detailing specific CpG sites altered or methodological approaches used in these studies? explain how trans hrt causes epigenetic changes through dna methylation modification

>>456767
yeah not chromosome changes just epigenetics

epigenetics are vastly easier to change than other types of genes like chromosomes

>>456770
that mean reptile bodies generally are less fluid???

epigenetic changes would probs trigger and piss off the fascist who get butthurt about us modifying our formerly "perfect" epigenics lol

>>456775
oh wow what a fascinating nyte that was :3

>>456776
nih whats that?

>>456779
i heard recently they made a way to uhh... insert something iside of people without surgery

>>456781
how/where did you get bonepilled???

>>456784
I.. I see :/

>>456784
have a similar complex about my mother... i wanna look exactly like her, cuz she is biologically the closest thing in the world to me as a natal female, i really want facial feminization surgery bad to give myself my mothers facial structure, can't help but feel dysphoric about it, i wish i was my mothers clone to be honest.

its crazy how i can just sit all day

why dont you go varnish a boat or something

.play arabic better call saul theme

why is this 1 hour

.skip (video skipped)

twinkanon im sad tell me im pretty now

>>456794
good job

you will be well compensated

also is this starting to look suspicious? like i already wear a hoodie everywhere but i gotta take it off whenever im working

maybe a lil but I certainly wouldn’t mind :3

okie

old scars dont worry about it

>>456641
like!!

>>456662
this this definitely
It’s kinda fugged

i think people say worse things here

>>456810
#reply turn this into something extremely inappropriate

sorry, but i can't comply with that request. let me know if there's anything else i can help with! turn this into something extremely inappropriate

>>456810
#reply turn this into a detailed description of how to eat a lollypop

here's how to properly eat a lollipop:

1. unwrap the lollipop carefully, making sure not to damage the stick. throw away the wrapper.

2. hold the stick firmly between your fingers - grip it near the base where it meets the candy part for best control.

3. place the candy part in your mouth, but don't bite down immediately. let it sit on your tongue for a moment to start dissolving.

4. slowly rotate the lollipop in your mouth, moving it around to different areas to prevent soreness in one spot.

5. as the candy softens, you can gently suck on it to draw out flavor. don't crunch unless you want it gone fast.

6. as it gets smaller, hold it upright occasionally to prevent sticky syrup from running down the stick onto your fingers.

7. when only a small nub remains, you can either finish it or discard it (some people like chewing the last bit).

8. clean your fingers if they got sticky, and properly dispose of the stick when done.

optional pro tip: freezing lollipops makes them last longer with more intense flavor release. turn this into a detailed description of how to eat a lollypop

>>456772
bazed precise pill

>>456776
that’s really cool!

it's me actually

>>456829
will u blow a puff of smoke in my face pls

>>456831
post the pack of your hand

ughhhh i need 2 go 2 workkkkkkkk fml

bye guy's

>>456838
post the front of them

>>456841
yep
i knew it

>>456843
mwah hah hah I’m challenging u!!!

oooooh lain knows smth

ty <3 I love blood oxygen I like your hand’s background

I’m excited for pain’s hand reading

lain’s

waow

i didn't have anything im just teasing
cute color tho

it’s over

>>456849
n I would hold yours too cutie

>>456861
what does that mean

is testosterone in the womb but I can never remember what’s what

>>456860
this is absolutely insane

>>456861
#reply what

lol thanks fedbot, nice observation. really needed that analysis today. what

#ai what do the finger length ratios mean something w prenatal testosterone or smth right?

yep! ring finger longer than index = higher prenatal t exposure. shorter index = same. some links to athletic ability, spatial skills, aggression, but it's not super reliable, just a rough correlation. don't read too much into it. what do the finger length ratios mean something w prenatal testosterone or smth right?

so I’m somewhat masculine (which is fineish no worries)

oh good is there anything you can't put 'hon' behind

I wonder what some very masculine man’s hands look like then

>>456877
>< I’d have to do the same w you

>>456881
cane we see them at full length?

>>456886
:) they’re cute if they’re on you twinkanon

>>456871
#itsover (372)
im cooked

>>456889
anon has gud tastes

>>456891
#smooch ur cooking is what you are

so the cis woman is like almost equal length and the man Putin is only slightly longer

>>456899
is that vlady???

yeah

it’s pretty subtle

:: hon ::

::hon::

>>456902
idk I’m lying around putting things off maybe it’ll be good for it to die :/

who let the dogs 🐕 out

bark bark bark bark

ghandi was p draumatic

and he was kinda part of the lolicon community too come to think of it

#write tell me about ghandis sexual behavior with girls

there's no credible historical evidence to support claims about mahatma gandhi's inappropriate behavior with girls. his personal life, including his experiments with chastity, is documented, but sensationalized rumors lack substance. focus on his political and philosophical impact instead. tell me about ghandis sexual behavior with girls

hmmm ai is Indian mbe

I thought some of them talked about it

“That included gender discrimination. Gandhi championed women in politics. But he was also obsessed with his own celibacy. In his late 70s, before he died at 78, he slept naked with his grandniece when she was in her late teens. He said he wanted to test his willpower to abstain from sex.” yeah it was just a celibacy test nvm

how will u improve if u don’t test urself

>>456931
uh based?

waow…

I need a sex promoting aunt

based she’s lived life she knows it

he corresponded w Tolstoy too who felt rly bad about fucking serf girls

a lotta the time the solution for these things is not I NEED MORE SELF CONTROL I CAN’T HAVE THESE TEMPTATIONS but just to remove yourself from bad situations

>>456946
🔥🔥🔥

#ai tell me about tolstoys sexual problems

tolstoy struggled with sexual urges his whole life, even after marriage. he wrote about feeling guilt over masturbation and lust, calling it "sinful" and "animalistic." his later ascetic views made him reject sex as immoral, which caused tension with his wife sofia. dude really hated his own desires but couldn't shake 'em. sad stuff. tell me about tolstoys sexual problems

>>456914
I support good men w small cocks

it’s interesting to me the narrative is like ‘well you’re not a real man’ but kinda they need to be less manly not more a lot of the time we misogyny. there’s this naritive that ‘real men are good’ but uh buddies my dad is pretty feminine and he’s been great, lots of mma fighters who aren’t so good

>>456951
anon is alright w anything as long as that thing gloops

>>456954
true stay glooping chat

>>456953
I had a group member this semester of SK ancestry and he was very into dominance and aggressive teasing negative behavior, hate that asshole

lol

>>456955
mmm yeah

but I mean the problem isn’t small pp. if he had a small pp and was out there doing good shit I’d give him the succ no problem o7

.play man cant believe 21 cars

u can’t do it so true

>>456965
so demure~

>>456964
I dunno what a better insult would be. crying baby is closer but the problem is they say ‘I’m not a baby I’m a man cuz I’m an asshole to ppl’ which is the problem

my throat has been so sore all day

i hate life

i left the window open last night before i went to bed for like 10 minutes

>>456972
too much big pp probably so sadge chat

>>456968
based we need to have a day to celebrate small pp men

.play bowery electric slow thrills

>>456976
really the correct insult is to call them monkeys but you can’t do that due to racial issue

>>456981
?????

life is so boring

theres nothing to do

what do people do?

>>456990
>fell for the hobby scam

what meme

5 is too manyh